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ELECTROSPRAY MASS-SPECTROMETRY [1 record]

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Record 1 2010-06-08

English

Subject field(s)
  • Genetics
  • Biochemistry
CONT

The coupling of isoelectric focusing on immobilized pH gradients (IPG) with electrospray-mass spectrometry (ES-MS) was applied to the characterization of proteins according to two different and important properties, such as net surface charge and molecular mass. From a technical point of view, these methods are complementary, since ES- MS requires ion-free samples as usually supplied by isoelectric focusing on IPGs.

French

Domaine(s)
  • Génétique
  • Biochimie
CONT

[...] la spectrométrie de masse tient une grande place dans la panoplie des techniques analytiques. Les méthodes d'ionisation, connues sous le nom d'ES-MS (Electrospray-Mass Spectrometry), de MALDI (Matrix Assisted Laser Ionization), permettent la caractérisation des protéines recombinantes, la détermination des modifications post-traductionnelles (glycolysation, acylation, phosphorilation...) de protéines de haut poids moléculaire (jusqu'à 300 kDa), et l'étude des complexes non covalents formés par le récepteur et son ligand.

Spanish

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